Submit Manuscript  

Article Details


Polyphenolic Compound from Vitis vinifera L. have Potential for the Alzheimer Disease Treatment

[ Vol. 14 , Issue. 7 ]

Author(s):

Soukup Ondrej, Stralkova Radomira, Mistova Tereza, Popov Marek, Hejtmankova Alena, Lachman Jaromir, Klimova Blanka and Kuca Kamil*   Pages 853 - 859 ( 7 )

Abstract:


Background: Grape marc and the wine cakes are known for high content of catechins, which have been reported to have many beneficial effects for human health. The most important are the effects on cardiovascular system. Also some potential to combat the Alzheimer┬┤s disease has been reported for catechins contained in a green or white tea.

Objective: To extract polyphenols from the wine grape seeds and try to characterize basic targets, which play an important role in AD.

Methods: Using HPLC method we have identified and assessed the content of various catechins in the wine cake and grape seeds extracts. Applying in vitro methodologies we have evaluated the potency of the extract to inhibit acetylcholinesterase and prolyl oligoesterase. We also assessed the effect on the cell viability (MTT test) and its antioxidant activity (DPPH test)

Results and Conclusion: It was shown that Pinot Gris, Pinot Noir and Hibernal are the varieties containing most of the polyphenolic compounds. Catechin followed by epicatechin and epigallocatechin were the most abundant polyphenols in the cake extract. We also report the biological activity of the extract with regard to a potential to improve cognitive decline associated with neurodegenerative disease like the Alzheimer┬┤s disease. Apart from the expected antioxidant activity, there is an interesting prolyl oligopeptidase affinity, which can be an added value for targeting the cognitive decline.

Keywords:

Alzheimer's disease, grape wine extract, prolyl oligopeptidase, oxidative stress, catechins, polyphenols.

Affiliation:

Biomedical Research Centre, University Hospital Hradec Kralove, Sokolska 581, 50005 Hradec Kralove, Viticulture Research Station Karlstejn, Crop Research Institute, Drnovska 507/73, 16106 Praha, Viticulture Research Station Karlstejn, Crop Research Institute, Drnovska 507/73, 16106 Praha, Department of Chemistry, Czech University of Life Sciences in Prague, Kamycka 129, 165 21 Prague 6-Suchdol, Department of Chemistry, Czech University of Life Sciences in Prague, Kamycka 129, 165 21 Prague 6-Suchdol, Department of Chemistry, Czech University of Life Sciences in Prague, Kamycka 129, 165 21 Prague 6-Suchdol, Faculty of Informatics and Management, University of Hradec Kralove, Rokitanskeho 62, 50003, Hradec Kralove, Biomedical Research Centre, University Hospital Hradec Kralove, Sokolska 581, Hradec Kralove

Graphical Abstract:



Read Full-Text article