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Illicium verum Extract Exhibited Anti-inflammatory Action in Rodents

[ Vol. 15 , Issue. 6 ]

Author(s):

Shagufta Khan*, Huma Aslam Bhatti*, Ghulam Abbas, Muhammad Ali Versiani, Shaheen Faizi, Salman Khalid Ahmed, Saad Jawaid, Anita Naushir Akbar Ali, Rabia Jamy, Fatima Shahid and Ahsana Dar Farooq   Pages 678 - 686 ( 9 )

Abstract:


Background: Illicium verum Hook. f. (Star anise) has been traditionally used for the treatment of abdominal colic, vomiting, pain, nervousness, insomnia and rheumatism.

Objective: The present study was conducted to investigate the anti-inflammatory properties of Illicium verum extracts.

Methods: Different extracts of I. verum (IV-Ext-1, IV-Ext-3 and IV-Ext-4) were screened for their anti-inflammatory potential using carrageenan-induced paw edema in mice. The mechanism of action was explored using paw edema tests (carrageenan, histamine, serotonin and prostaglandins) in rats and anti-oxidant activity (DPPH assay). Reverse phase HPLC was used for chemical characterization of extracts.

Results: The IV-Ext-3 was found to be the most potent anti-inflammatory agent (38% inhibition) using carrageenan-induced paw edema. The anti-inflammatory effect was observed during both the initial (IC50: 260 mg/kg) and late phases (IC50: 200 mg/kg). Similar IC50 of 200 mg/kg was observed in both phases of histamine-induced paw edema. However, the extract failed to cause 50% inhibition in serotonin-induced edema (IC25: 400 mg/kg) and was also ineffective against Prostaglandin- E2 induced edema. Furthermore, the IV-Ext-3 appeared to be an effective antioxidant (IC50: 12 µg/ml). The HPLC chromatogram of IV-Ext-3 showed the presence of anti-inflammatory constituents such as shikimic acid (1.6%) and anethole (0.25%), 3, 4-dihydroxybenzoic acid (0.003%) and p-hydroxy methyl benzoate (0.117%) along with some polar hydroxyl derivatives of benzoic acids.

Conclusion: Among all the extracts of I. verum, IV-Ext-3 was found to be the most effective antiinflammatory agent which can be attributed to multiple anti-inflammatory constituents, antihistamine and antioxidant potentials.

Keywords:

Star anise, anti-histamine, prostaglandins synthesis inhibitor, antioxidant, anti-inflammatory, edema.

Affiliation:

Department of Biological and Biomedical Sciences, Aga Khan University Hospital, Medical College, Karachi, HEJ Research Institute of Chemistry, International Center for Chemical and Biological Sciences, University of Karachi, Karachi-75270, HEJ Research Institute of Chemistry, International Center for Chemical and Biological Sciences, University of Karachi, Karachi-75270, Department of Chemistry, Federal Urdu University of Arts, Sciences and Technology, Gulshan-e-Iqbal, Karachi, HEJ Research Institute of Chemistry, International Center for Chemical and Biological Sciences, University of Karachi, Karachi-75270, Department of Chemistry, Federal Urdu University of Arts, Sciences and Technology, Gulshan-e-Iqbal, Karachi, Department of Emergency Medicine, King Fahad Medical City, Riyadh, Department of Anesthesiology, University of Arkansas for Medical Sciences, 4301 W Markham St., Little Rock, AR, Department of Biological and Biomedical Sciences, Aga Khan University Hospital, Medical College, Karachi, Department of Biological and Biomedical Sciences, Aga Khan University Hospital, Medical College, Karachi, Faculty of Eastern Medicine, Hamdard University, Karachi

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