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The Impact of Antioxidants from the Diet on Breast Cancer Cells Monitored by Raman Microspectroscopy

[ Vol. 16 , Issue. 2 ]

Author(s):

Paula Sofia Coutinho Medeiros, Ana Lúcia Marques Batista de Carvalho*, Cristina Ruano, Juan Carlos Otero and Maria Paula Matos Marques   Pages 127 - 137 ( 11 )

Abstract:


Background: The impact of the ubiquitous dietary phenolic compound p-coumaric acid on human breast cancer cells was assessed, through a multidisciplinary approach: Combined biological assays for cytotoxicity evaluation and biochemical profiling by Raman microspectroscopic analysis in cells.

Methods: Para-coumaric acid was shown to exert in vitro chemoprotective and antitumor activities, depending on the concentration and cell line probed: a significant anti-invasive ability was detected for the triple-negative MDA-MB-231 cells, while a high pro-oxidant effect was found for the estrogen- dependent MCF-7 cells. A striking cell selectivity was obtained, with a more noticeable outcome on the triple-negative MDA-MB-231 cell line.

Results: The main impact on the cellular biochemical profile was verified to be on proteins and lipids, thus justifying the compound´s anti-invasive effect and chemoprotective ability.

Conclusion: p-Coumaric acid was thus shown to be a promising chemoprotective/chemotherapeutic agent, particularly against the low prognosis triple-negative human breast adenocarcinoma.

Keywords:

Dietary antioxidants, p-coumaric acid, breast cancer cells, chemoprotective/chemotherapeutic, raman microspectroscopy, antitumor activities.

Affiliation:

R&D Unit "Quimica-Fisica Molecular", Department of Chemistry, Faculty of Science and Technology, University of Coimbra, 3004-535 Coimbra, R&D Unit "Quimica-Fisica Molecular", Department of Chemistry, Faculty of Science and Technology, University of Coimbra, 3004-535 Coimbra, Department of Physical-Chemistry, Faculty of Science, University of Malaga, Unidad Asociada CSIC, Malaga, Department of Physical-Chemistry, Faculty of Science, University of Malaga, Unidad Asociada CSIC, Malaga, R&D Unit "Quimica-Fisica Molecular", Department of Chemistry, Faculty of Science and Technology, University of Coimbra, 3004-535 Coimbra

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