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Investigation Of Reducing Omniscan Toxicity Using Intracellular And Targeted N-Acetylcysteine Lysine Complex

[ Vol. 16 , Issue. 9 ]

Author(s):

Hadi Hejazinia, Elham Poonaki, Mitra Elmi, Mohammad Esfandiar, Vala Vahedian Boroujeni, Artin Assadi, Seyed Esmaeil Sadat Ebrahimi, Morteza Pirali Hamedani and Mehdi Shafiee Ardestani*   Pages 1006 - 1019 ( 14 )

Abstract:


Background: The main issue is finding the most efficient method in the treatment of cancer in terms of early and accurate diagnostic. One of the most modern diagnostic techniques is imaging methods. The accuracy and detection speed of MRI and CT SCAN are high.

Methods: The most important complication of iodinated contrast agents in medical imaging is severe renal toxicity Nephrogenic Systemic Fibrosis (NSF). In order to reduce the cytotoxicity of kidney cells caused by the usage of iodized contrast agents a complex agent should be designed. The two drugs which have been used for the synthesis of this compound are L -lysine amino acid and NAcetyl- Cysteine (NAC).

Results: The synthesis of this complex due to two dimer molecules with each other and NAC greatly a helper for an antioxidant activity and L-lysine amino acid helps in drug entry into the cells. However, helping for an antioxidant activity heavily reinforce and eventually will successfully reduce the cytotoxicity. When its exposure to HEK 293 cell line (P<0.05). The reduction in toxicity at the dosage of 100 µM has been showed as the greatest reduction. The amount of renal toxicity was reported 40% in Omniscan.

Conclusion: Omniscan was tested when iodinated contrast medium was combined with the synthesized 2NAC-LYS-OMNISCAN complex and the human embryonic kidney 293 (HEK293) cell line. Then, the cytotoxicity was reduced to 10 %. On the other hand, the viability increased from 60 % to 90 %, or in other words, the cytotoxicity was reduced from 40 % to 10 %.

Keywords:

Cancer, N-acetylcysteine, lysine, NMR, reduced toxicity, increased permeability of omniscan.

Affiliation:

Department of Radiopharmacy, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Department of Biotechnology, I.A.U of Damghan, Damghan, Department of Biomedical Sciences, Faculty of Medicine and Health Sciences, Macquarie University, North Ryde, NSW, Department of Radiopharmacy, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, School of Pharmacy, International Campus, Tehran University of Medical Sciences, Tehran, Centre for Bioactive Discovery in Health and Ageing, School of Science and Technology, University of New England, Armidale, School of Pharmacy, International Campus, Tehran University of Medical Sciences, Tehran, Department of Medicinal Chemistry, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Department of Radiopharmacy, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran

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