Seyed Ahmad Emami, Hadi Al-Najjar, Javad Asili, Maryam Sadat Habibi, Amirhossein Sahebkar and Zahra Tayarani-Najaran* Pages 563 - 569 ( 7 )
Background: Nepeta species is a rich source of phytochemicals with multiple pharmacological activity. The aim of the present study was to study the cytotoxicity and pro-apoptotic activity of Nepeta binaloudensis different extracts and essential oil on prostate (PC3 and DU-145) and breast cancer (MCF-7) cell lines.
Methods: To explore that cytotoxicity and apoptosis inducing activity, resazurin assay, propidium iodide (PI) staining of cells and western blotting analysis of PARP, Bax and Cyt c were performed.
Results: Estrogen receptor (ER)-positive PC3, and MCF-7 cells were found to be more sensitive to N. binaloudensis in comparison with hormone-refractory DU-145 cells. Methylene chloride extract and the essential oil of N. binaloudensis showed the highest cytotoxicity (IC50 < 100 µg/mL). The amount of cytochrome C and Bax protein increased, and PARP was cleaved with methylene chloride fraction and essential oil of the plant which confirms induction of apoptosis.
Conclusion: The present results suggested cytotoxic and apoptotic activity of N. binaloudensis on ER-positive breast cancer cells. Further phytochemical investigations are required to find phytochemical( s) with potential anti-tumor activity in this plant.
Nepeta binaloudensis, Lamiaceae, cytotoxicity, apoptosis, essential oil, propidium iodide (PI).
Department of Traditional Pharmacy, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Department of Pharmacy, College of Health Science, Public Authority for Applied Education and Training (PAAET), Kuwait Health Science Pharmacy, Adailiya, Department of Pharmacognosy, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Medical Toxicology Research Center, Mashhad University of Medical Sciences, Mashhad, Biotechnology Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Biotechnology Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad