Submit Manuscript  

Article Details


Design, Synthesis and Bioactivity of Core 1 O-glycan and its Derivative on Human Gut Microbiota

[ Vol. 16 , Issue. 12 ]

Author(s):

Huanhuan Qu*, Baixue Li, Jingyi Yang, Huaiwen Liang, Meixia Li and Kan Ding*   Pages 1348 - 1353 ( 6 )

Abstract:


Background: Disaccharide core 1 (GalĪ²1-3GalNAc) is a common O-glycan structure in nature. Biochemical studies have confirmed that the formation of the core 1 structure is an important initial step in O-glycan biosynthesis and it is of great importance for human body.

Objective: Our study will provide meaningful and useful sights for O-glycan synthesis and their bioassay. And all the synthetic glycosides would be used as intermediate building blocks in the scheme developed for oligosaccharide construction.

Methods: In this article, we firstly used chemical procedures to prepare core 1 and its derivative, and a novel disaccharide was efficiently synthesized. The structures of the synthesized compounds were elucidated and confirmed by 1H NMR, 13C NMR and MS. Then we employed three human gut symbionts belonging to Bacteroidetes, a predominantphyla in the distal gut, as models to study the bioactivity of core 1 and its derivative on human gut microbiota.

Results: According to our results, both core 1 and derivative could support the growth of B. fragilis, especially the core 1 derivative, while failed to support the growth of B. thetaiotaomicron and B. ovatus.

Conclusion: This suggested that the B. fragilis might have the specificity glycohydrolase to cut the glycosidic bond for acquiring monosaccharide.

Keywords:

Bioactivity, core 1, derivative, human gut microbiota, O-glycan, synthesis.

Affiliation:

Glycochemistry & Glycobiology Lab, Key Laboratory of Receptor Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, 555 Zu Chong Zhi Road, Pudong, Shanghai 201203, Department of Basic Mediclal Sciences, Chengdu University of Traditional Chinese Medicine, Chengdu 610075, Department of Pharmacy, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, Department of Pharmacy, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, Glycochemistry & Glycobiology Lab, Key Laboratory of Receptor Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, 555 Zu Chong Zhi Road, Pudong, Shanghai 201203, Glycochemistry & Glycobiology Lab, Key Laboratory of Receptor Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, 555 Zu Chong Zhi Road, Pudong, Shanghai 201203

Graphical Abstract:



Read Full-Text article